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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene has been observed in lots of tumors, which results in chromosome instability, and therefore could lead to tumorigenesis. This protein was found to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a vital function in endorsing cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, happen to be described. Two extra splice variants were being noted but in-depth nucleotide sequence details will not be yet offered.

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Blocking peptides are peptides that bind particularly towards the target antibody and block antibody binding. These peptide generally contains the epitope regarded through the antibody.

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This guide outlines additional attainable optimization of mobile and immunoassay parameters to be certain the best possible success are...

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This tutorial outlines even further probable optimization of mobile and immunoassay parameters to make sure the best possible final results are...

Antibodies bound to the blocking peptide now not bind into the epitope to the target protein. By comparing the staining from your blocked antibody compared to the antibody alone, you can see which staining is certain.

Target Information Cyclin E belongs into the really conserved cyclin household, whose users are characterized by a extraordinary periodicity in protein abundance from the mobile cycle. Cyclins functionality as regulators of CDK kinases. Different cyclins exhibit unique expression and degradation styles which add for the temporal coordination of every mitotic event. Cyclin E types a posh with and capabilities to be a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates on the G1-S section boundary and it is degraded as cells read more development via S period.

The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...

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Clicking the pictures or hyperlinks will redirect you to a web site hosted by BenchSci that provides 3rd-occasion scientific information. Neither the articles nor the BenchSci know-how and procedures for collection have already been evaluated by us; we have been providing them as-is and without warranty of any kind, which include for use or software with the Thermo Fisher Scientific goods offered.